cd14 fc recombinant protein  (R&D Systems)

Journal: PLoS ONE

Article Title: Comparative Analysis of Hepatic CD14 Expression between Two Different Endotoxin Shock Model Mice: Relation between Hepatic Injury and CD14 Expression

doi: 10.1371/journal.pone.0053692

Figure Lengend Snippet: Plasma was collected from CAR-primed C57BL/6 mice that were administered with LPS at the indicated time. Soluble CD14 in the plasma was detected as follows: Each plasma sample was diluted 20 folds with saline, and then subjected to SDS-PAGE and subsequent western blotting. Then, sCD14 on the membrane was detected using anti-mouse CD14 polyclonal antibody.

Article Snippet: Recombinant mouse CD14 in the culture supernatant was purified by Ni-nitrilotriacetic acid agarose (Qiagen, Basel, Switzerland) chromatography using elution buffer containing imidazole (250 mM).

Techniques: SDS Page, Western Blot

Journal: PLoS ONE

Article Title: Comparative Analysis of Hepatic CD14 Expression between Two Different Endotoxin Shock Model Mice: Relation between Hepatic Injury and CD14 Expression

doi: 10.1371/journal.pone.0053692

Figure Lengend Snippet: Liver specimens were collected from CAR-primed C57BL/6 mice after LPS administration at 12 h or D-gal N -primed C57BL/6 mice after LPS administration at 6 h. These hepatic tissues were immunohistochemically studied by staining with the biotinylated anti-mouse CD14 monoclonal antibody 4C1. (A) Following priming with CAR (5 mg/mouse), these mice were injected with LPS (20 μg/mouse). (B) The mice were simultaneously injected with D-gal N (20 mg/mouse) and LPS (0.5 μg/mouse). (C) The mice were injected with saline. CD14 expression was observed (arrow) in the area surrounding the bile duct (white arrowhead). Magnification: ×200.

Article Snippet: Recombinant mouse CD14 in the culture supernatant was purified by Ni-nitrilotriacetic acid agarose (Qiagen, Basel, Switzerland) chromatography using elution buffer containing imidazole (250 mM).

Techniques: Staining, Injection, Expressing

Journal: PLoS ONE

Article Title: Comparative Analysis of Hepatic CD14 Expression between Two Different Endotoxin Shock Model Mice: Relation between Hepatic Injury and CD14 Expression

doi: 10.1371/journal.pone.0053692

Figure Lengend Snippet: Bile samples were collected from the gallbladder 12 h after CAR-primed C57BL/6 mice were injected with LPS (A), after D-gal N -primed C57BL/6 mice were injected with LPS (B), and from the negative control (C). Each sample was diluted with saline, and then subjected to SDS-PAGE and subsequent western blot analysis. The expression of sCD14 on the nitro cellulose membrane was detected using an anti-mouse CD14 polyclonal antibody as a probe.

Article Snippet: Recombinant mouse CD14 in the culture supernatant was purified by Ni-nitrilotriacetic acid agarose (Qiagen, Basel, Switzerland) chromatography using elution buffer containing imidazole (250 mM).

Techniques: Injection, Negative Control, SDS Page, Western Blot, Expressing

Journal: PLoS ONE

Article Title: Comparative Analysis of Hepatic CD14 Expression between Two Different Endotoxin Shock Model Mice: Relation between Hepatic Injury and CD14 Expression

doi: 10.1371/journal.pone.0053692

Figure Lengend Snippet: The immunohistochemistry of the mice hepatic tissues was studied by staining with the biotinylated anti-mouse CD14 monoclonal antibody (10 μg/mL) 4C1. The tissues were collected from CAR-primed C57BL/6 mice that were injected with LPS at 12 h or from the control mice and the transfected plasma was collected from the CAR-primed mice that were injected with LPS at 12 h or from the control mice. C57BL/6 mice were injected with LPS at 6 h. (A) The mice were primed with CAR (5 mg/mouse) and injected with LPS (20 μg/mouse). (B) The liver of the mice that intravenously received the plasma prepared from CAR-primed and LPS-injected mice. (C) The liver of an untreated mouse was used as control. (D) The livers were extirpated from the mice that received control plasma, prepared from control mice. CD14 expression (arrow) was observed in the area surrounding the bile duct (white arrowhead). Magnification: ×200.

Article Snippet: Recombinant mouse CD14 in the culture supernatant was purified by Ni-nitrilotriacetic acid agarose (Qiagen, Basel, Switzerland) chromatography using elution buffer containing imidazole (250 mM).

Techniques: Immunohistochemistry, Staining, Injection, Transfection, Expressing

Journal: PLoS ONE

Article Title: Comparative Analysis of Hepatic CD14 Expression between Two Different Endotoxin Shock Model Mice: Relation between Hepatic Injury and CD14 Expression

doi: 10.1371/journal.pone.0053692

Figure Lengend Snippet: The gene expression of CD14 in the mice liver was determined by in situ hybridization with CD14 sense and antisense probes. (A) Liver specimens from CAR-primed endotoxin shock-induced mice stained using CD14 antisense probe (B) Liver specimens from CAR-primed endotoxin shock-induced mice stained using CD14 sense probe (C) Liver specimens from mice injected with LPS (20 ng/mouse) stained using CD14 antisense probe (D) Liver specimens from mice injected with LPS (20 ng/mouse) stained using CD14 sense probe. Arrow represents CD14 mRNA expression. Magnification: ×200.

Article Snippet: Recombinant mouse CD14 in the culture supernatant was purified by Ni-nitrilotriacetic acid agarose (Qiagen, Basel, Switzerland) chromatography using elution buffer containing imidazole (250 mM).

Techniques: Expressing, In Situ Hybridization, Staining, Injection